plotQA.FCS {rflowcyt} | R Documentation

## Create a scatterplot summaryzing one (or two) parameter(s) for several FCS objects stored in a plate

### Description

Create a scatterplot summaryzing one (or two) parameter(s) for several FCS objects stored in a plate. The points are colored according to their position in the plate (row or column number.)

### Usage

    plotQA.FCS(data,varpos=c(1,2),FUN1=IQR,FUN2=NULL,col="row",nrow=8,ncol=12,ind=c(1:96),labeling=TRUE,...)

### Arguments

`data` | a list of fluorescent data from one (or more) FCS object(s) or a cytoset
`varpos` | the numerical column variable position of the data of the FCS object
`FUN1` | function to summaryze the distribution of the data, e.g. mean, median, IQR, MODE
`FUN2` | function to summaryze the distribution of the data e.g. mean, median, IQR, MODE
`col` | character vector either "row" or "col"
`nrow` | numeric, number of rows per plate
`ncol` | numeric, number of columns per plate
`ind` | numeric vector, index of the wells to be plotted
`labeling` | logical, draw plate position (default=TRUE)
`...` | any other arguments are passed to the `plot` function

### Value

None.

### Author(s)

Nolwenn Le Meur

### See Also

`plot`

### Examples

      ##Example I:
     data(flowcyt.data)

     ##Draw a scatterplot of the median values
     ##of the Foward scatter and the Side scatter parameters
     ##of each FCS file. The files correspond to samples store in a 96 well plate.
     plotQA.FCS(flowcyt.data,varpos=c(1,2),FUN1=median,nrow=8,ncol=10,ind=c(1:80),col="row",pch="*",labeling=FALSE,xlim=c(0,300),ylim=c(0,300),main="FSCmedian vs.SSCmedian by row",xlab="SSC median",ylab="FSC median")

     ##Example II:
     ##Draw a a scatterplot of the mode and IQR values for the Foward scatter
     ##of each FCS file.
     plotQA.FCS(flowcyt.data,varpos=c(1),FUN1=IQR,FUN2=MODE,nrow=8,ncol=10,ind=c(1:80),col="col",pch="*",labeling=TRUE,xlim=c(0,300),ylim=c(0,300),xlab="FSC mode",ylab="FSC IQR",main="FSC IQR vs.FSC mode by column")

[Package rflowcyt version 1.16.0 Index]