showgate.FCS {rflowcyt} | R Documentation

## Showing the gate and the datapoints within the gate on a prevous plot

### Description

On an exisiting plot, the gate specified will be plotted and the datapoints lying within the gating range will be colored (default is the color purple).

### Usage

    showgate.FCS(data.mat, gatingrange, Index,
           type = c("uniscut", "biscut", "bidcut", "bipcut"),
           IndexValue.In = 1,
           coltype = 12, pchtype = 8,
           biscut.quadrant = c("+/-","-/-", "+/+", "-/+"))

### Arguments

`data.mat` | the data to be gated: univariate case single column of values: a (m X 1) data vector where m is the number of cells/rows bivariate case matrix of two column variables: a (m X 2) data matrix where m is the number or cells/rows
`gatingrange` | gating threshold range in one of the following formats for each type of gating: "uniscut" univariate single cut; gatingrange = x1 will select/include all points >= x1 , x1 is numeric value "bidcut" bivariate double cut: gatingrange = c(x1, x2, y1,y2), a numeric vector of lowerbound, upperbound cutoffs for x and y variables "biscut" bivariate single cut:gatingrange = c(x1,y1), a numeric vector of the cutoffs for x and y variables "bipcut" bivariate polygonal cut: polygonal thresholds for an n-sided polygon with gatingrange = cbind(c(x1, x2, ..., xn, x1), c(y1, y2, ..., yn, y1)), a vector of vectors which denote the outer points of the polygonal vertices
`Index` | a vector of 0's and 1's denoting the selection of row observations of 'data.mat'
`type` | character string of the type of cut/gating: "uniscut" univariate single cut: selects datapoints that are greater than or equal to the cutoff value denoted in gatingrange "bidcut" bivariate double cut: selects datapoints in the central rectangle formed by two vertical lines (x variable cutoffs) and two horizontal lines (y variable cutoffs) "biscut" bivariate single cut: cuts graph into quadrants (selects datapoints in the quadrant denoted by biscut.quadrant) "bipcut" bivariate polygonal cut: selects the datapoints in a polygon
`IndexValue.In` | The value of 'Index' to be selected; default is 1
`coltype` | a character string or a numerical value describing the option for the color of the data point inside the gating range
`pchtype` | a character string or a numerical value describing the option for the point size and type of data point inside the gating range
`biscut.quadrant` | character string value denoting the (x,y) quadrant that is to be selected; Values are one of the following: "$+$/$+$" selects the upper right quadrant, where x is positive and y is positive "$-$/$+$" selects the upper left quadrant, where x is negative and y is positive "$+$/$-$" selects the lower right quadrant, where x is positive and y is negative "$-$/$-$" selects the lower left quadrant, where x is negative and y is negative

### Value

The gating range or gate will be displayed and the data points within the gating range will be colored.

### Note

The coloring in of data points may take a while to process. The gate selection can only be shown using rectangular binning of the image plots using `ContourScatterPlot`. The `showgate.FCS` does not work with hexagonal binning.

### Author(s)

A.J. Rossini and J.Y. Wan

### See Also

`FHCRC.HVTNFCS`,`VRC.HVTNFCS`, `plotvar.FCS`,`createGate`, `icreateGate`

### Examples

      if (interactive()){
      if (require(rfcdmin)){
        ## obtaining the FCS objects from VRC data
        if ( !(is.element("unst.1829", objects())
                        & is.element("st.1829", objects())) ){
          data(VRCmin)
        }

        ## univariate plot
        plotvar.FCS(unst.1829, type="uni", varpos=1, plotType=hist)
        ## show cut off at 350
        showgate.FCS(unst.1829@data[,1], type="uniscut", gatingrange=350)
        ## show different cutoff at 500
        showgate.FCS(unst.1829@data[,1], type="uniscut", gatingrange=500,
                     coltype="green")

        ## bivariate plot : rectanglar bins in which the gate can be shown
        plotvar.FCS(unst.1829, type="bi", varpos=c(1,2), hexbin.CSPlot=FALSE)
        ## show cutoff at 275 to 600 for both variables
        ## may take a while
        ## create the gate index as the first column entry of the "gate" matrix
        unst.1829.gt<-createGate(unst.1829, varpos=1:2, type="bidcut",
              gatingrange=c(275, 600, 275, 600))

        ## show the gate
        showgate.FCS(unst.1829.gt@data[,c(1,2)], unst.1829.gt@gate[,1],
           type="bidcut", gatingrange=c(275, 600, 275, 600))

        }
      }

[Package rflowcyt version 1.16.0 Index]