Bscore {cellHTS} | R Documentation

## B score normalization

### Description

Correction of plate and spatial effects of the data `xraw` of a `cellHTS` object using the B score method. Using this method, a two-way median polish is fitted in a per-plate basis to account for row and column effects. Optionally, the obtained residuals within each plate can be further divided by their median absolute deviations to standardize for plate-to-plate variability. Optionally, a transformation to z-scores can be performed.

### Usage

    Bscore(x, what="xraw", adjustPlateMedian = TRUE, scale = TRUE, save.model = FALSE)

### Arguments

`x` | a `cellHTS` object that has already been configured. See details.
`what` | a character indicating the slot of `x` to consider: `"xraw"` (default) or `"xnorm"`.
`adjustPlateMedian` | a logical value indicating whether the estimated average of each plate should also be substracted to the raw intensity values.
`scale` | a logical value indicating if the per-plate model residuals should be further scaled by their variance. See details.
`save.model` | a logical value specifying whether the per-plate models should be saved, and given as output. See details.

### Details

The normalization is performed in a per-plate fashion using the B score method. This function can be called direclty, or indirectly, using `normalizePlates` or `summarizeChannels`. In the B score method, the residual r_{ijp} of the measurement for row i and column j on the p-th plate is obtained by fitting a two-way median polish, in order to account for both row and column effects within the plate:

r_{ijp} = y_{ijp} - yest_{ijp} = y_{ijp} - (mu_p + R_{ip} + C_{jp})

y_{ijp} is the measurement value in row i and column j of plate p (taken from `x$xraw`), and yest_{ijp} is the corresponding fitted value. This is defined as the sum between the estimated average of the plate (mu_p), the estimated systematic offset for row i (R_{ip}), and the systematic offset for column j (C_{jp}).

If `scale=TRUE`, for each plate p, each of the obtained residual values r_{ijp}'s are divided by the median absolute deviation of the residuals in plate p (MAD_p), giving the B score value:

Bscore_{ijp} = r_{ijp}/MAD_p

If `adjustPlateMedian` is set to `FALSE`, the estimated overall plate average (mu_p) is not removed from the intensity values y_{ijp}'s.

If `save.model=TRUE`, the models residuals (r_{ijp}'s), row and column offsets and overall offsets are stored in the slots `residuals`, `rowcol.effects` and `overall.effects` of the `cellHTS` object `x`.

### Value

An object of class `cellHTS`, which is a copy of the argument `x`, plus an additional slot `xnorm` containing the normalized data. This is an array of the same dimensions as `xraw`. Furthermore, if `save.model=TRUE`, the slots `residuals`, `rowcol.effects`, and `overall.effects` (only if `adjustPlateMedian` was also set to `TRUE`) are added to `x`. The latter slots are arrays with the same dimension as `x$xraw`, except the `overall.effects`, which have dimensions `1 x nr Plates x nr Replicates x nr Channels`.
Moreover, the processing status of the `cellHTS` object is updated in the slot `state` to `x$state["normalized"]=TRUE`.

### Author(s)

Ligia Braz ligia@ebi.ac.uk

### References

Brideau, C., Gunter, B., Pikounis, B. and Liaw, A. (2003) Improved statistical methods for hit selection in high-throughput screening, J. Biomol. Screen 8, 634–647.

Malo, N., Hanley, J.A., Cerquozzi, S., Pelletier, J. and Nadon, R. (2006) Statistical practice in high-throughput screening data analysis, Nature Biotechn 24(2), 167–175.

### See Also

`medpolish`, `plotSpatialEffects`, `normalizePlates`, `summarizeChannels`

### Examples

        data(KcViabSmall)
        x <- KcViabSmall
        x <- Bscore(x, save.model = TRUE)
        ## identical result, but calling Bscore function from "normalizePlates"
        xopt <- normalizePlates(x, normalizationMethod="Bscore", save.model = TRUE)
        all(x$xnorm==xopt$xnorm, na.rm=TRUE)

[Package cellHTS version 1.10.0 Index]