bg.correct {affy} | R Documentation

## Background Correction

### Description

Background corrects probe intensities in an object of class `AffyBatch`.

### Usage

    bg.correct(object, method, ...)

    bg.correct.rma(object,...)
    bg.correct.mas(object, griddim)
    bg.correct.none(object, ...)

### Arguments

`object` | An object of class `AffyBatch`.
`method` | A `character` that defines what background correction method will be used. Available methods are given by `bg.correct.methods`.
`griddim` | grid dimension used for mas background estimate. The array is divided into griddm equal parts. Default is 16.
`...` | arguments to pass along to the engine function.

### Details

The name of the method to apply must be double-quoted. Methods provided with the package are currently: begin{itemize}

- bg.correct.none: returns `object` unchanged.
- bg.correct.chipwide: noise correction as described in a `white paper' from Affymetrix.
- bg.correct.rma: the model based correction used by the RMA expression measure. end{itemize}

  They are listed in the variable `bg.correct.methods`. The user must supply the word after "bg.correct", i.e none, subtractmm, rma, etc...

  More details are available in the vignette.

  `bg.correct.rma2` gives access to the older background method that was used by the `rma` function in version 1.1 bioconductor releases. For the 1.2 release `rma` performs the same background adjustment as `bg.correct.rma`. Unless you wish to duplicate older results in which case you should use `bg.correct.rma`.

  Details on the internal fucntions used by `bg.correct.rma` are in `bg.adjust`.

  ### Value

  An `AffyBatch` for which the intensities have been background adjusted. For some methods (RMA), only PMs are corrected and the MMs remain the same.

  ### Examples

          data(affybatch.example)

          ##bgc will be the bg corrected version of affybatch.example
          bgc <- bg.correct(affybatch.example,method="rma")

          ##This plot shows the tranformation
          plot(pm(affybatch.example)[,1],pm(bgc)[,1],log="xy",
              main="PMs before and after background correction")

  [Package affy version 1.16.0 Index]