isoNorm {isomiRs} | R Documentation

## Normalize count matrix

### Description

This function normalizes raw count matrix using `DESeq2::rlog()` function from DESeq2::DESeq2-package.

### Usage

    isoNorm(ids, formula = NULL, maxSamples = 50)

### Arguments

`ids` | Object of class IsomirDataSeq.
`formula` | Formula that will be used for normalization.
`maxSamples` | Maximum number of samples to use with `DESeq2::rlog()`, if not `limma::voom()` is used.

### Value

IsomirDataSeq object with the normalized count matrix in a slot. The normalized matrix can be access with `counts(ids, norm=TRUE)`.

### Examples

    data(mirData)
    ids <- isoCounts(mirData, minc=10, mins=6)
    ids <- isoNorm(mirData, formula=~condition)
    head(counts(ids, norm=TRUE))

[Package isomiRs version 1.12.0 Index]