BeadFlowFrame-class {flowBeads} | R Documentation

## BeadFlowFrame

### Description

Extension of `flowFrame` specific for bead data.

The constructor take as arguments the FCS file and the file containing the MEF values of the beads on the different detector channels

### Usage

      BeadFlowFrame(fcs.filename, bead.filename)

### Arguments

`fcs.filename` | The file name of the FCS to load. File is loaded with the `read.FCS` function.
`bead.filename` | The file name of the MEF configuration files indicating the type of beads in the FCS file. The bead.file is read with read.csv.

### Slots

`fcs.filename`:

  The file name of the FCS file from which to read.

`bead.filename`:

  The file name of the bead config file.

`beads.mef`:

  The `data.frame` containing the MEF of the bead populations on different channels.

`trans`:

  The transform f to linearise the fluorescence.

`inv.trans`:

  The inverse transform of f^{-1}.

[Package flowBeads version 1.22.0 Index]