plotExplainedVariance {decompTumor2Sig} | R Documentation

## Plot the explained variance as a function of the number of signatures

### Description

'plotExplainedVariance()' plots the explained variance of a single tumor genome's mutation patterns as a function of the number of signatures (increasing subsets of signatures) used for decomposition. For each number K of signatures, the highest variance explained by possible subsets of K signatures will be plotted (full or greedy search, see below). This can help to evaluate what minimum threshold for the explained variance can be used to decompose tumor genomes with the function `decomposeTumorGenomes`.

### Usage

    plotExplainedVariance(genome, signatures, minExplainedVariance=NULL,
    minNumSignatures=2, maxNumSignatures=NULL, greedySearch=FALSE)

### Arguments

`genome` | (Mandatory) The mutation load of a single genome in Alexandrov- of Shiraishi-format, i.e. as vector or matrix. The format must be the same as the one used for the `signatures` (see below).
`signatures` | (Mandatory) The list of signatures (vectors, data frames or matrices) which are to be evaluated. Each of the list objects represents one mutational signature. Vectors are used for Alexandrov signatures, data frames or matrices for Shiraishi signatures.
`minExplainedVariance` | (Optional) If a numeric value between 0 and 1 is specified, the plot highlights the smallest subset of signatures which is sufficient to explain at least the specified fraction of the variance of the genome's mutation patterns. If, for example, `minExplainedVariance` is 0.99 the smallest subset of signatures that explains at least 99% of the variance will be highlighted.
`minNumSignatures` | (Optional) The plot will be generated only for K>=`minNumSignatures`.
`maxNumSignatures` | (Optional) The plot will be generated only for K<=`maxNumSignatures`.
`greedySearch` | (Optional) If `greedySearch` is set to `TRUE` then not all possible combinations of `minNumSignatures` to `maxNumSignatures` signatures will be checked. Instead, first all possible combinations for exactly `minNumSignatures` will be checked to select the best starting set, then iteratively the next best signature will be added (maximum increase in explained variability) until `maxNumSignatures` is reached). NOTE: while this is only an approximation, it is highly recommended for large sets of signatures (>15)!

### Value

Returns (or draws) a plot of the explained variance as a function of the number of signatures.

### Author(s)

Rosario M. Piro
Freie Universitaet Berlin
Maintainer: Rosario M. Piro
E-Mail: <rmpiro@gmail.com> or <r.piro@fu-berlin.de>

### References

http://rmpiro.net/decompTumor2Sig/
Krueger, Piro (2018) decompTumor2Sig: Identification of mutational signatures active in individual tumors. BMC Bioinformatics (accepted for publication).
Krueger, Piro (2017) Identification of Mutational Signatures Active in Individual Tumors. NETTAB 2017 - Methods, Tools & Platforms for Personalized Medicine in the Big Data Era, October 16-18, 2017, Palermo, Italy. PeerJ Preprints 5:e3257v1, 2017.

### See Also

`decompTumor2Sig`
`decomposeTumorGenomes`
`computeExplainedVariance`

### Examples

    ### get 15 pre-processed Shiraishi signatures computed (object 'signatures')
    ### from 435 tumor genomes Alexandrov et al (PMID: 23945592)
    ### using the pmsignature package
    sfile <- system.file("extdata",
             "Alexandrov_PMID_23945592_435_tumors-pmsignature-15sig.Rdata",
             package="decompTumor2Sig")
    load(sfile)

    ### load preprocessed breast cancer genomes (object 'genomes') from
    ### Nik-Zainal et al (PMID: 22608084)
    gfile <- system.file("extdata",
             "Nik-Zainal_PMID_22608084-genomes-Shiraishi_5bases_trDir.Rdata",
             package="decompTumor2Sig")
    load(gfile)

    ### plot the explained variance for 2 to 6 signatures of the first genome
    plotExplainedVariance(genomes[[1]], signatures,
             minExplainedVariance=0.98, minNumSignatures=2, maxNumSignatures=6)

[Package decompTumor2Sig version 2.0.0 Index]