postTreatment {RJMCMCNucleosomes} | R Documentation

## A post-treatment function to merge closely positioned nucleosomes, from the same chromosome, identified by the `rjmcmc` function.

### Description

A helper function which merges closely positioned nucleosomes to rectify the over splitting and provide a more conservative approach. Beware that each chromosome must be treated separatly.

### Usage

    postTreatment(reads, seqName = NULL, resultRJMCMC, extendingSize = 74L,
      chrLength)

### Arguments

`reads` | a `GRanges` containing forward and reverse reads. Beware that the start position of a reverse read is always higher that the end positition.
`seqName` | a `character` string containing the label of the chromosome, present in the `GRanges` object, that will be used. The `NULL` value is accepted when only one seqname is present in the `GRanges`; the only seqname present will be used. Default: `NULL`.
`resultRJMCMC` | an object of `class` "rjmcmcNucleosomes" or "rjmcmcNucleosomesMerge", the information about nucleosome positioning for an entire chromosome or a region that must be treated as one unit.
`extendingSize` | a positive `numeric` or a positive `integer` indicating the size of the consensus region used to group closeley positioned nucleosomes.The minimum size of the consensus region is equal to twice the value of the `extendingSize` parameter. The numeric will be treated as an integer. Default: 74.
`chrLength` | a positive `numeric` or a positive `integer` indicating the length of the current chromosome. The length of the chromosome is used to ensure that the consensus positions are all located inside the chromosome.

### Value

a `GRanges`, the updated nucleosome positions. When no nucleosome is present, `NULL` is returned.

### Author(s)

Pascal Belleau, Astrid Deschenes

### Examples

    ## Loading dataset
    data(reads_demo_02)

    ## Nucleosome positioning, running both merge and split functions
    result <- rjmcmc(reads = reads_demo_02,
                seqName = "chr_SYNTHETIC", nbrIterations = 1000,
                lambda = 2, kMax = 30, minInterval = 146,
                maxInterval = 490, minReads = 3, vSeed = 11)

    ## Before post-treatment
    result

    ##Post-treatment function which merged closely positioned nucleosomes
    postResult <- postTreatment(reads = reads_demo_02,
                        seqName = "chr_SYNTHETIC", result, 100, 73500)

    ## After post-treatment
    postResult

[Package RJMCMCNucleosomes version 1.8.0 Index]