Package: CRISPRseek
Type: Package
Title: Design of target-specific guide RNAs in CRISPR-Cas9,
        genome-editing systems
Version: 1.24.0
Date: 2019-02-15
Encoding: UTF-8
Author: Lihua Julie Zhu, Benjamin R. Holmes, Hervé Pagès, Michael Lawrence, Isana Veksler-Lublinsky, Victor Ambros, Neil Aronin and Michael Brodsky
Maintainer: Lihua Julie Zhu <julie.zhu@umassmed.edu>
Depends: R (>= 3.0.1), BiocGenerics, Biostrings
Imports: parallel, data.table, seqinr, S4Vectors (>= 0.9.25), IRanges,
        BSgenome, BiocParallel, hash
Suggests: RUnit, BiocStyle, BSgenome.Hsapiens.UCSC.hg19,
        TxDb.Hsapiens.UCSC.hg19.knownGene, org.Hs.eg.db
Description: The package includes functions to find potential guide RNAs for input target sequences, optionally filter guide RNAs without restriction enzyme cut site, or without paired guide RNAs, genome-wide search for off-targets, score, rank, fetch flank sequence and indicate whether the target and off-targets are located in exon region or not. Potential guide RNAs are annotated with total score of the top5 and topN off-targets, detailed topN mismatch sites, restriction enzyme cut sites, and paired guide RNAs. This package leverages Biostrings and BSgenome packages.
License: GPL (>= 2)
LazyLoad: yes
biocViews: ImmunoOncology, GeneRegulation, SequenceMatching, CRISPR
git_url: https://git.bioconductor.org/packages/CRISPRseek
git_branch: RELEASE_3_9
git_last_commit: 196e02b
git_last_commit_date: 2019-05-02
Date/Publication: 2019-05-02
NeedsCompilation: no
Packaged: 2019-05-03 02:47:58 UTC; biocbuild
Built: R 3.6.0; ; 2019-05-03 13:08:35 UTC; windows
