plotGenomicRegions {ASpli} | R Documentation

## Create genomic regions coverage plots

### Description

Graphic representation of coverage and junctions is useful to complement the results of differential usage of bins and junction and differential expression analysis.

Function plotGenomicRegions allow to create plots for multiple conditions for bins and genes. Each individual plot can only correspond to a single gene or bin, but can contain many panels for different experimental conditions.

### Usage

      plotGenomicRegions( features, x, genomeTxDb, targets, xIsBin = TRUE,
        layout = 'auto', colors = 'auto', plotTitles = 'auto', sashimi = FALSE,
        zoomOnBins = FALSE, deviceOpt = NULL, highLightBin = TRUE, outfolder = NULL,
        outfileType = 'png', mainFontSize = 24, annotationHeight = 0.2,
        annotationCol = 'black', annotationFill = 'gray', annotationColTitle = 'black',
        preMergedBAMs = NULL, useTransparency = FALSE, tempFolder = 'tmp',
        avoidReMergeBams= FALSE, verbose = TRUE )

### Arguments

`features` | An ASpliFeatures object, generated with `binGenoms` function.
`x` | A character vector with the names of bins or genes to plot. To plot into a window is recommended that the length of x be one.
`genomeTxDb` | A TxDb object with the annotation of reference genome.
`targets` | A data frame containing sample, bam files and experimental factor columns
`xIsBin` | A logical value that indicates if values in x corresponds to gene names or bin names.
`layout` | A character with value 'auto' or a character matrix with condition names arranged with the desired layout of the panels in plots. The dimensions of layout matrix, colors matrix and plotTitles matrices must be the same. Matrix can have NA values, however, the height of the panels corresponding to that column are modified to occupy the total height. The default value is 'auto'.
`colors` | A character containing value 'auto' or containing colors strings, or a character matrix with color names arranged with the layout specified in layout argument. The dimensions of layout matrix, colors matrix and plotTitles matrices must be the same. The default value is 'auto'.
`plotTitles` | A character containing value 'auto', or a character matrix with titles for each panel arranged with the layout specified in layout argument. The dimensions of layout matrix, colors matrix and plotTitles matrices must be the same. The default value is 'auto'.
`sashimi` | A logical value that specifies that a sashimi plot for junctions must be included into each panel. The default value is FALSE.
`zoomOnBins` | A FALSE logical value or a double value between 0 and 1. If value is FALSE then the genomic range to be plotted correspond to the complete gene, otherwise the genomic range is that the size of the bin corresponds to a fraction equals to zoomOnBins value of the total and is centered in the bin. Is used only when xIsBin is TRUE. The default value is FALSE.
`deviceOpt` | A named list of arguments to be passed to the graphic device used to plot. This allow to further customization of the plot. The default value is an empty list.
`highLightBin` | A logical value that indicates if the bin should be highlighted. The default value is TRUE.
`outfolder` | NULL or a character vector representing a folder path that will be used to save the plot images. If the folder doesn't exists it is created. If NULL, the plot is made in a window. The default value is NULL. For each bin, a single image file is generated. The name of the file is the name of the bin, added with a '.gr.' string, and the file extension at the end. If the name of the bin contains invalid character for a file name, those will be replaced by an underscore character.
`outfileType` | A character value the specifies the file format of the plot to be created. Is used only when outfolder is not NULL. Accepted values are 'png', 'jpeg', 'bmp', 'tiff', 'pdf'. Each value is the graphic device used to create the image. The default value is 'png'.
`mainFontSize` | A numeric value specifying the size of the main title. The default value is 24.
`annotationHeight` | A double value specifying the proportion of the total height used to represent the gene model. The default value is 0.2.
`annotationCol` | A character value that specifies the color of the borders of bars in gene model representation. The default value is 'black'.
`annotationFill` | A character value that specifies the color of the filling of bars in gene model representation. The default value is 'gray'.
`annotationColTitle` | A character value that specifies the color of text in gene model representation. The default value is 'black'.
`preMergedBAMs` | A one column data frame that associates a condition, specified in the row name, with a character value representing the path of bam file with the reads for that condition. This bam file is typically generated by merging the bam files of all replicates for that condition. The default value is NULL, this specifies that not merged bam files are used, instead on-the-fly read extraction and merging is done from the bam files specified in the targets argument.
`useTransparency` | A logical value that specifies if transparency will be used to generate the plots, this leads to better looking plot, however not all graphic devices support transparency. The default value is FALSE.
`tempFolder` | A character value specifying the path to store intermediate files produced while extracting and merging reads from bam files. It is only used when preMergedBAMs arguments is NULL. The files created are not automatically removed after plotting because can be reused to create a new plot of the same genes or bins with different graphic options. The default value is 'tmp', that means a new 'tmp' folder in the current working folder.
`avoidReMergeBams` | A logical value specifying that extraction and merging of bam files will be avoided. This is only meaningful when the extraction and merging of the same set of genes and bins was done in the previous execution of plotGenomicRegions function. The default value is FALSE.
`verbose` | A logical value specifying that detailed information about the execution will be informed to the user. The default value is TRUE.

### Author(s)

Estefania Mancini, Javier Iserte, Marcelo Yanovsky, Ariel Chernomoretz

### See Also

`Devices`, `pdf`, `png`, `bmp`, `jpeg`, `tiff`

### Examples

      # Create a transcript DB from gff/gtf annotation file.
      # Warnings in this examples can be ignored.
      library(GenomicFeatures)
      genomeTxDb <- makeTxDbFromGFF( system.file('extdata','genes.mini.gtf',
                                     package="ASpli") )

      # Create an ASpliFeatures object from TxDb
      features <- binGenome( genomeTxDb )

      # Define bam files, sample names and experimental factors for targets.
      bamFileNames <- c( "A_C_0.bam", "A_C_1.bam", "A_C_2.bam",
                         "A_D_0.bam", "A_D_1.bam", "A_D_2.bam" )
      targets <- data.frame(
                   row.names = paste0('Sample_',c(1:6)),
                   bam = system.file( 'extdata', bamFileNames, package="ASpli" ),
                   factor1 = c( 'C','C','C','D','D','D'),
                   stringsAsFactors = FALSE )

      # Plot a single bin to a window
      plotGenomicRegions(
        features,
        'GENE01:E002',
        genomeTxDb,
        targets,
        sashimi = FALSE,
        colors = '#AA4444',
        annotationHeight = 0.1,
        tempFolder = 'tmp',
        verbose = TRUE ,
        avoidReMergeBams = FALSE,
        useTransparency = FALSE )

      # plot two bins to pdf files.
      plotGenomicRegions(
        features, c( 'GENE01:E002', 'GENE02:E002' ),
        genomeTxDb,
        targets,
        layout = matrix( c( 'C', 'D'), ncol = 1),
        colors = matrix( c( '#663243', '#363273'), ncol = 1),
        plotTitles = matrix( c( 'C condition', 'D condition'), ncol = 1),
        sashimi = FALSE,
        mainFontSize = 12,
        annotationHeight = 0.1,
        tempFolder = 'tmp',
        verbose = TRUE ,
        avoidReMergeBams = FALSE,
        useTransparency = TRUE,
        outfolder = '.',
        outfileType = 'pdf',
        deviceOpt = list( height = 6, width = 5, paper = 'a4r' ) )

[Package ASpli version 1.10.0 Index]