straightColLines {CONFESS} | R Documentation

## straightColLines

### Description

It identifies the vertical BF image characteristic lines.

### Usage

    straightColLines(img, pattern.search, cut = seq(0.3, 1.5, 0.02), ImgLimits,
      chip.type)

### Arguments

`img` | Data matrix. The BF image data matrix.
`pattern.search` | Integer. A cutoff to find horizontal and vertical lines on the chip.
`cut` | Integer. A normalized signal cutoff above which a pixel is considered as foreground.
`ImgLimits` | Integer. It instructs the algorithm to find spots in a certain central image area. For example, for a 512 x 512 image with cutSides = 50, it will search for spots in the central area [ImgLimits:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix.
`chip.type` | Character string. It specifies the type of Fluidigm chip to be analyzed.

### Value

Estimated vertical BF characteristic lines.

[Package CONFESS version 1.8.0 Index]