spotCenter {CONFESS} | R Documentation

## spotCenter

### Description

It estimates a series of spot statistics on each channel.

### Usage

    spotCenter(img, foregroundCut, howbig, ImgLimits)

### Arguments

`img` | Data matrix. The channel image data.
`foregroundCut` | Numeric vector. The binary segmentation image analysis cutoffs for normalized image data. Pixels with normalized signals higher than the cutoff belong to foreground.
`howbig` | Integer. An user defined value of the minimum expected spot size.
`ImgLimits` | a cutoff that determines where the spot is supposed to be found.

### Value

A list of spot coordinate estimates

[Package CONFESS version 1.8.0 Index]