SampleAssayPlot-class {iSEE} | R Documentation

## The SampleAssayPlot panel

### Description

The SampleAssayPlot is a panel class for creating a RowDotPlot where the y-axis represents the expression of a sample of interest, using the `assay` values of the SummarizedExperiment. It provides slots and methods for specifying the sample and what to plot on the x-axis, as well as a method to actually create a data.frame containing those pieces of data in preparation for plotting.

### Slot overview

The following slots control the values on the y-axis:

- `YAxisSampleName`, a string specifying the name of the sample to plot on the y-axis. If `NA`, defaults to the first column name of the SummarizedExperiment object.

- `Assay`, string specifying the name of the assay to use for obtaining expression values. Defaults to `"logcounts"` in `getPanelDefault`, falling back to the name of the first valid assay (see `?".cacheCommonInfo,DotPlot-method"` for the definition of validity).

- `YAxisSampleSource`, string specifying the encoded name of the transmitting panel to obtain a single selection that replaces `YAxisSampleName`. Defaults to `"---"`, i.e., no transmission is performed.

- `YAxisSampleDynamicSource`, a logical scalar indicating whether `x` should dynamically change its selection source for the y-axis. Defaults to `FALSE` in `getPanelDefault`.

The following slots control the values on the x-axis:

- `XAxis`, string specifying what should be plotted on the x-axis. This can be any one of `"None"`, `"Sample name"`, `"Row data"` or `"Row selection"`. Defaults to `"None"`.

- `XAxisColumnData`, string specifying which column of the `colData` should be shown on the x-axis, if `XAxis="Column data"`. Defaults to the first valid `colData` field (see `?".refineParameters,ColumnDotPlot-method"` for details).

- `XAaxisSampleName`, string specifying the name of the sample to plot on the x-axis, if `XAxis="Sample name"`. Defaults to the first column name.

- `XAxisSampleSource`, string specifying the encoded name of the transmitting panel to obtain a single selection that replaces `XAxisSampleName`. Defaults to `"---"`, i.e., no transmission is performed.

- `XAxisSampleDynamicSource`, a logical scalar indicating whether `x` should dynamically change its selection source for the x-axis. Defaults to `FALSE` in `getPanelDefault`.

In addition, this class inherits all slots from its parent ColumnDotPlot, DotPlot and Panel classes.

### Constructor

`SampleAssayPlot(...)` creates an instance of a SampleAssayPlot class, where any slot and its value can be passed to `...` as a named argument.

### Supported methods

In the following code snippets, `x` is an instance of a SampleAssayPlot class. Refer to the documentation for each method for more details on the remaining arguments.

For setting up data values:

- `.refineParameters(x, se)` replaces any `NA` values in `XAxisSampleName` and `YAxisSampleName` with the first column name; any `NA` value in `Assay` with the first valid assay name; and any `NA` value in `XAxisColumnData` with the first valid column metadata field. This will also call the equivalent ColumnDotPlot method for further refinements to `x`. If no columns or assays are present, `NULL` is returned instead.

For defining the interface:

- `.defineDataInterface(x, se, select_info)` returns a list of interface elements for manipulating all slots described above.

- `.panelColor(x)` will return the specified default color for this panel class.

For monitoring reactive expressions:

- `.createObservers(x, se, input, session, pObjects, rObjects)` sets up observers for all slots described above and in the parent classes. This will also call the equivalent ColumnDotPlot method.

For defining the panel name:

- `.fullName(x)` will return `"Sample assay plot"`.

For creating the plot:

- `.generateDotPlotData(x, envir)` will create a data.frame of sample assay values in `envir`. It will return the commands required to do so as well as a list of labels.

For managing selections:

- `.singleSelectionSlots(x)` will return a list specifying the slots that can be updated by single selections in transmitter panels, mostly related to the choice of sample on the x- and y-axes. This includes the output of the method for the parent RowDotPlot class.

- `.multiSelectionInvalidated(x)` returns `TRUE` if the x-axis uses multiple row selections, such that the point coordinates may change upon updates to upstream selections in transmitting panels. Otherwise, it dispatches to the RowDotPlot method.

For documentation:

- `.definePanelTour(x)` returns an data.frame containing a panel-specific tour.

### Author(s)

Aaron Lun

### See Also

ColumnDotPlot, for the immediate parent class.

### Examples

    #################
    # For end-users #
    #################

    x <- SampleAssayPlot()
    x[["XAxis"]]
    x[["Assay"]] <- "logcounts"
    x[["XAxisRowData"]] <- "stuff"

    ##################
    # For developers #
    ##################

    library(scater)
    sce <- mockSCE()
    sce <- logNormCounts(sce)

    old_assay_names <- assayNames(sce)
    assayNames(sce) <- character(length(old_assay_names))

    # Spits out a NULL and a warning if no assays are named.
    sce0 <- .cacheCommonInfo(x, sce)
    .refineParameters(x, sce0)

    # Replaces the default with something sensible.
    assayNames(sce) <- old_assay_names
    sce0 <- .cacheCommonInfo(x, sce)
    .refineParameters(x, sce0)

[Package iSEE version 2.6.0 Index]