iTRAQ4 {MSnbase} | R Documentation

## iTRAQ 4-plex set

### Description

This instance of class `"ReporterIons"` corresponds to the iTRAQ 4-plex set, i.e the 114, 115, 116 and 117 isobaric tags. In the iTRAQ5 data set, an unfragmented tag, i.e reporter and attached isobaric tag, is also included at MZ 145. These objects are used to plot the reporter ions of interest in an MSMS spectra (see `"Spectrum2"`) as well as for quantification (see `quantify`).

### Usage

    iTRAQ4
    iTRAQ5
    iTRAQ8
    iTRAQ9

### References

Ross PL, Huang YN, Marchese JN, Williamson B, Parker K, Hattan S, Khainovski N, Pillai S, Dey S, Daniels S, Purkayastha S, Juhasz P, Martin S, Bartlet-Jones M, He F, Jacobson A, Pappin DJ. "Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents." Mol Cell Proteomics, 2004 Dec;3(12):1154-69. Epub 2004 Sep 22. PubMed PMID: 15385600.

### See Also

`TMT6`.

### Examples

    iTRAQ4
    iTRAQ4[1:2]

    newReporter <- new("ReporterIons",
                       description="an example",
                       name="my reporter ions",
                       reporterNames=c("myrep1","myrep2"),
                       mz=c(121,122),
                       col=c("red","blue"),
                       width=0.05)
    newReporter

[Package MSnbase version 2.20.1 Index]