KRAS mutant and wild type droplet counts and Poisson estimates.

Description

A data frame of droplet counts using the standard Cluster classification from KRASdata. Each row corresponds to a well/sample and the columns PP, PN, NP and NN show how many droplets were in each cluster. The remaining rows were calculated from the counts figures except for the InputAmount column.

KRAScountsWellCol is the same data frame but has a Well column instead of named rows.

KRAScountsQS is a data frame imported from a CSV created by Bio-Rad's QuantaSoft.

Usage

data(KRAScounts)

data(KRAScountsWellCol)

data(KRAScountsQS)

Format

A data frame where each row corresponds to a well/sample.

Details

The data frame was created by:

krasPlate <- ddpcrPlate(wells=KRASdata)
KRAScounts <- plateSummary(krasPlate, cMethod="Cluster")
onesVector <- c(1, 1, 1)
runAmount <- c(64 * onesVector, 16 * onesVector, 4 * onesVector, onesVector)
KRAScounts$InputAmount <- runAmount

KRAScountsWellCol <- KRAScounts
KRAScountsWellCol$Well <- rownames(KRAScounts)
KRAScountsWellCol <- KRAScountsWellCol[, c(18,1:17)]
rownames(KRAScountsWellCol) <- NULL

Value

A data frame.

Author(s)

From the KRASdata dataset created by Mahmood Ayub, mahmood.ayub@cruk.manchester.ac.uk