R: Analysis of steady state fluorescence flow cytometry

steadyState {flowTime}

R Documentation

Analysis of steady state fluorescence flow cytometry

Description

Generates a data frame which can be used to visualize and analyze steady state flow cytometry data. Steady state in this case means that

Usage

steadyState(flowset, gated = FALSE, ploidy = "diploid", only = "singlets")

Arguments

`flowset`	your flowSet to be analyzed
`gated`	`boolean` is the data already gated?
`ploidy`	`character` gate to subset your flowset based on the ploidy of you strains
`only`	`character` which population of events to analyze, 'yeast', singlets', or 'doublets'?

Value

a data frame containing all of the selected subset of events from the original flowSet

Examples

dat <- read.flowSet(path = system.file("extdata", "ss_example",
package = "flowTime"), alter.names = TRUE)
annotation <- read.csv(system.file("extdata", "ss_example.csv",
package = "flowTime"))
dat <- annotateFlowSet(dat, annotation, mergeBy = "name")
loadGates(gatesFile = 'SORPGates')
steadyState(dat, gated = FALSE, ploidy = "diploid", only = "singlets")

[Package flowTime version 1.12.0 Index]