vandesompele             package:SLqPCR             R Documentation

_D_a_t_a _s_e_t _o_f _V_a_n_d_e_s_o_m_p_e_l_e _e_t _a_l (_2_0_0_2)

_D_e_s_c_r_i_p_t_i_o_n:

     This data set was used in Vandesompele et al (2002) to demonstrate
     normalization  of real-time quantitative RT-PCR data by geometric
     averaging of housekeeping genes.

_U_s_a_g_e:

     data(vandesompele)

_F_o_r_m_a_t:

     A data frame with 85 observations on the following 10 variables
     which stand for  expression data of ten commonly used housekeeping
     genes

     '_A_C_T_B' actin, beta

     '_B_2_M' beta-2-microglobulin

     '_G_A_P_D' glyceraldehyde-3-phosphate dehydrogenase

     '_H_M_B_S' hydroxymethylbilane synthase

     '_H_P_R_T_1' hypoxanthine phosphoribosyltransferase 1

     '_R_P_L_1_3_A' ribosomal protein L13a

     '_S_D_H_A' succinate dehydrogenase complex subunit A

     '_T_B_P' TATA box binding protein

     '_U_B_C' ubiquitin C

     '_Y_W_H_A_Z' tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
          activation protein, zeta polypeptide

_D_e_t_a_i_l_s:

     The row names of this data set indicate the various human tissues
     which were investigated. 

     _B_M 9 normal bone-marrow samples

     _P_O_O_L 9 normal human tissues from pooled organs (heart, brain,
          fetal brain, lung, trachea, kidney, mammary gland, small
          intestine and uterus)

     _F_I_B 20 short-term cultured normal fibroblast samples from
          different individuals

     _L_E_U 13 normal leukocyte samples

     _N_B 34 neuroblastoma cell lines (independently prepared in
          different labs from different patients)

_S_o_u_r_c_e:

     The data set was obtained from <URL:
     http://genomebiology.com/content/supplementary/gb-2002-3-7-research0034-s1.txt>

_R_e_f_e_r_e_n_c_e_s:

     Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002).
     Accurate  normalization of real-time quantitative RT-PCR data by
     geometric averaging  of multiple internal control genes.  Genome
     Biology 2002. 3(7):research0034.1-0034.11. <URL:
     http://genomebiology.com/2002/3/7/research/0034/>

_E_x_a_m_p_l_e_s:

     data(vandesompele)
     str(vandesompele)
     rownames(vandesompele)

