| SPADE.addClusterToFCS | Annotate observations in FCS file with cluster assignment |
| SPADE.addDensityToFCS | Annotate FCS file with local density of each observation |
| SPADE.annotateGraph | Add attributes to graph |
| SPADE.annotateMarkers | Compute marker medians, coefficient of variations and counts for clusters |
| SPADE.density | Annotate FCS file with local density of each observation |
| SPADE.downsampleFCS | Downsample observcations in a FCS file according to density parameter |
| SPADE.driver | SPADE workflow driver |
| SPADE.FCSToTree | Cluster and build minimum spanning tree from data in FCS files |
| SPADE.flattenAnnotations | Flatten list of annotations to matrix |
| SPADE.installPlugin | Install CytoSPADE Cytoscape plugin |
| SPADE.layout.arch | Generate coordinates for plotting graphs |
| SPADE.markerMedians | Compute marker medians, coefficient of variations and counts for clusters |
| SPADE.plot.trees | Plot trees with annotated vertices |
| SPADE.read.FCS | Read an FCS File |
| SPADE.transform.FCS | Annotate FCS file with local density of each observation |
| SPADE.write.graph | Writing the graph to a file in some format |