normLoess {sights} | R Documentation

## Normalization by loess method

### Description

Apply loess normalization to data

### Usage

    normLoess(dataMatrix, plateRows, plateCols, dataRows = NULL,
      dataCols = NULL)

### Arguments

`dataMatrix` | Data frame or numeric matrix. Columns are plates, and rows are plate wells.
`plateRows, plateCols` | Number of rows/columns in plate.
`dataRows, dataCols` | Optional integer vector. Indicate which row/column numbers from the dataMatrix should be normalized. If NULL then all rows/columns from the dataMatrix are used.

### Details

Loess normalization adjusts each well by the fitted row and column values generated by calculating the loess curve for each row and column.

### Value

Numeric matrix of normalized data in the same format as dataMatrix

### Note

For information on how to arrange your dataset for dataMatrix, please see (`ex_dataMatrix`)

### References

Baryshnikova et al. (2010). Quantitative analysis of fitness and genetic interactions in yeast on a genome scale. Nature Methods, 7(12), 1017-1024.

### See Also

Other normalization methods: `normMedFil`, `normRobZ`, `normR`, `normSPAWN`, `normZ`

### Examples

    ## load dataset
    data(ex_dataMatrix)

    ## apply Loess method
    ex_normMatrix <- normLoess(dataMatrix = ex_dataMatrix, dataCols = 5:10,
    plateRows = 8, plateCols = 10)

[Package sights version 1.6.0 Index]