geneFreq {LymphoSeq} | R Documentation

## Gene frequencies

### Description

Creates a data frame of VDJ gene counts and frequencies.

### Usage

    geneFreq(productive.nt, locus = "VDJ", family = FALSE)

### Arguments

`productive.nt` | A list of one or more data frames of productive sequences generated by the LymphoSeq function productiveSeq where the parameter aggregate is set to "nucleotide".
`locus` | A character vector indicating which VDJ genes to include in the output. Available options include "VDJ", "DJ", "VJ", "DJ", "V", "D", or "J".
`family` | A Boolean value indicating whether or not family names instead of gene names are used. If TRUE, then family names are used and if FALSE, gene names are used.

### Value

Returns a data frame with the sample names, VDJ gene name, count, and % frequency of the V, D, or J genes (each gene frequency should add to 100% for each sample).

### Examples

    file.path <- system.file("extdata", "TCRB_sequencing", package = "LymphoSeq")

    file.list <- readImmunoSeq(path = file.path)

    productive.nt <- productiveSeq(file.list = file.list, aggregate = "nucleotide")

    geneFreq(productive.nt, locus = "VDJ", family = FALSE)

    # Create a heat map of V gene usage
    vfamilies <- geneFreq(productive.nt, locus = "V", family = TRUE)

    require(reshape)

    vfamilies <- reshape::cast(vfamilies, familyName ~ samples, value = "frequencyGene", sum)

    rownames(vfamilies) <- as.character(vfamilies$familyName)

    vfamilies$familyName <- NULL

    RedBlue <- grDevices::colorRampPalette(rev(RColorBrewer::brewer.pal(11, "RdBu")))(256)

    require(pheatmap)

    pheatmap::pheatmap(vfamilies, color = RedBlue, scale = "row")

    # Create a word cloud of V gene usage
    vgenes <- geneFreq(productive.nt, locus = "V", family = FALSE)

    require(wordcloud)

    wordcloud::wordcloud(words = vgenes[vgenes$samples == "TRB_Unsorted_83", "geneName"],
       freq = vgenes[vgenes$samples == "TRB_Unsorted_83", "frequencyGene"],
    	  colors = RedBlue)

    # Create a cumulative frequency bar plot of V gene usage
    vgenes <- geneFreq(productive.nt, locus = "V", family = FALSE)

    require(ggplot2)

    ggplot2::ggplot(vgenes, aes(x = samples, y = frequencyGene, fill = geneName)) +
      geom_bar(stat = "identity") +
      theme_minimal() +
      scale_y_continuous(expand = c(0, 0)) +
      guides(fill = guide_legend(ncol = 3)) +
      labs(y = "Frequency (%)", x = "", fill = "") +
      theme(axis.text.x = element_text(angle = 90, vjust = 0.5, hjust = 1))

[Package LymphoSeq version 1.8.0 Index]