FilterLowCounts {NOISeq} | R Documentation

## Methods to filter out low count features

### Description

Function to filter out the low count features according to three different methods.

### Usage

    filtered.data(dataset, factor, norm = TRUE, depth = NULL, method = 1, cv.cutoff = 100, cpm = 1, p.adj = "fdr")

### Arguments

`dataset` | Matrix or data.frame containing the expression values for each sample (columns) and feature (rows).
`factor` | Vector or factor indicating which condition each sample (column) in dataset belongs to.
`norm` | Logical value indicating whether the data are already normalized (TRUE) or not (FALSE).
`depth` | Sequencing depth of samples (column totals before normalizing the data). Depth only needs to be provided when method = 3 and norm = TRUE.
`method` | Method must be one of 1,2 or 3. Method 1 (CPM) removes those features that have an average expression per condition less than cpm value and a coefficient of variation per condition higher than cv.cutoff (in percentage) in all the conditions. Method 2 (Wilcoxon) performs a Wilcoxon test per condition and feature where in the null hypothesis the median expression is 0 and in the alternative the median is higher than 0. Those features with p-value greater than 0.05 in all the conditions are removed. Method 3 (Proportion test) performs a proportion test on the counts per condition and feature (or pseudo-counts if data were normalized) where null hypothesis is that the feature relative expression (count proportion) is equal to cpm/10^6 and higher than cpm/10^6 for the alternative. Those features with p-value greater than 0.05 in all the conditions are removed.
`cv.cutoff` | Cutoff for the coefficient of variation per condition to be used in method 1 (in percentage).
`cpm` | Cutoff for the counts per million value to be used in methods 1 and 3.
`p.adj` | Method for the multiple testing correction. The same methods as in the p.adjust function in stats package can be chosen: "holm", "hochberg", "hommel", "bonferroni", "BH", "BY", "fdr", "none".

### Author(s)

Sonia Tarazona

### Examples

    ## Simulate some count data
    datasim = matrix(sample(0:100, 2000, replace = TRUE), ncol = 4)

    ## Filtering low counts (method 1)
    myfilt1 = filtered.data(datasim, factor = c("cond1", "cond1", "cond2", "cond2"), norm = FALSE, depth = NULL, method = 1, cv.cutoff = 100, cpm = 1)

    ## Filtering low counts (method 2)
    myfilt2 = filtered.data(datasim, factor = c("cond1", "cond1", "cond2", "cond2"), norm = FALSE, method = 2)

    ## Filtering low counts (method 3)
    myfilt3 = filtered.data(datasim, factor = c("cond1", "cond1", "cond2", "cond2"), norm = FALSE, method = 3, cpm = 1)

[Package NOISeq version 2.34.0 Index]