melting {rmelting} | R Documentation

## Compute melting temperature of a nucleic acid duplex

### Description

Compute the enthalpy and entropy of helix-coil transition, and then the melting temperature of a nucleic acid duplex with the MELTING 5 software (Le Novère, 2001; Dumousseau et al., 2012).

### Usage

    melting(sequence, comp.sequence = NULL,
            nucleic.acid.conc,
            hybridisation.type = c("dnadna", "rnarna", "dnarna",
                                   "rnadna", "mrnarna", "rnamrna"),
            Na.conc, Mg.conc, Tris.conc, K.conc,
            dNTP.conc, DMSO.conc, formamide.conc,
            size.threshold = 60, force.self = FALSE, correction.factor,
            method.approx = c("ahs01", "che93", "che93corr",
                              "schdot", "owe69", "san98",
                              "wetdna91", "wetrna91", "wetdnarna91"),
            method.nn = c("all97", "bre86", "san04", "san96", "sug96",
                          "tan04", "fre86", "xia98", "sug95", "tur06"),
            method.GU = c("tur99", "ser12"),
            method.singleMM = c("allsanpey", "tur06", "zno07", "zno08", "wat11"),
            method.tandemMM = c("allsanpey", "tur99"),
            method.single.dangle = c("bom00", "sugdna02", "sugrna02", "ser08"),
            method.double.dangle = c("sugdna02", "sugrna02", "ser05", "ser06"),
            method.long.dangle = c("sugdna02", "sugrna02"),
            method.internal.loop = c("san04", "tur06", "zno07"),
            method.single.bulge.loop = c("tan04", "san04", "ser07" ,"tur06"),
            method.long.bulge.loop = c("san04", "tur06"),
            method.CNG = c("bro05"),
            method.inosine = c("san05", "zno07"),
            method.hydroxyadenine = c("sug01"),
            method.azobenzenes = c("asa05"),
            method.locked = c("mct04"),
            correction.ion = c("ahs01", "kam71", "marschdot",
                               "owc1904", "owc2004", "owc2104",
                               "owc2204", "san96", "san04", "schlif",
                               "tanna06", "tanna07", "wet91",
                               "owcmg08", "tanmg06", "tanmg07",
                               "owcmix08", "tanmix07"),
            method.Naeq = c("ahs01", "mit96", "pey00"),
            correction.DMSO = c("ahs01", "cul76", "esc80", "mus81"),
            correction.formamide = c("bla96", "lincorr"))

### Arguments

`sequence` | Sequence (5' to 3') of one strand of the nucleic acid duplex as a character string (Note: Uridine and thymidine are not considered as identical).
`comp.sequence` | Complementary sequence (3' to 5') of the nucleic acid duplex as a character string.
`nucleic.acid.conc` | Concentration of the nucleic acid strand (M or mol L-1) in excess as a numeric value.
`hybridisation.type` | The hybridisation type. Either `"dnadna"`, `"rnarna"`, `"dnarna"`, `"rnadna"`, `"mrnarna"` or `"rnamrna"` (see Hybridisation type options).
`Na.conc` | Concentration of Na ions (M) as a positive numeric value (see Ion and agent concentrations).
`Mg.conc` | Concentration of Mg ions (M) as a positive numeric value (see Ion and agent concentrations).
`Tris.conc` | Concentration of Tris ions (M) as a positive numeric value (see Ion and agent concentrations).
`K.conc` | Concentration of K ions (M) as a positive numeric value (see Ion and agent concentrations).
`dNTP.conc` | Concentration of dNTP (M) as a positive numeric value (see Ion and agent concentrations).
`DMSO.conc` | Concentration of DMSO (%) as a positive numeric value (see Ion and agent concentrations).
`formamide.conc` | Concentration of formamide (M or % depending on correction method) as a positive numeric value (see Ion and agent concentrations).
`size.threshold` | Sequence length threshold to decide approximative or nearest-neighbour approach for computation. Default is 60.
`force.self` | logical. Enforces that `sequence` is self complementary and `complementary sequence` is not required (seed Self complementary sequences). Default is `FALSE`.
`correction.factor` | Correction factor to be used to modulate the effect of the nucleic acid concentration (`nucleic.acid.conc`) in the computation of melting temperature (see Correction factor for nucleic acid concentration).
`method.approx` | Specify the approximative formula to be used for melting temperature calculation for sequences of length greater than `size.threshold`. Either `"ahs01"`, `"che93"`, `"che93corr"`, `"schdot"`, `"owe69"`, `"san98"`, `"wetdna91"`, `"wetrna91"` or `"wetdnarna91"` (see Approximative formulas).
`method.nn` | Specify the nearest neighbor model to be used for melting temperature calculation for perfectly matching sequences of length lesser than `size.threshold`. Either `"all97"`, `"bre86"`, `"san04"`, `"san96"`, `"sug96"`, `"tan04"`, `"fre86"`, `"xia98"`, `"sug95"` or `"tur06"` (see Perfectly matching sequences).
`method.GU` | Specify the nearest neighbor model to compute the contribution of GU base pairs to the thermodynamic of helix-coil transition. Either `"tur99"` or `"ser12"` (see GU wobble base pairs effect).
`method.singleMM` | Specify the nearest neighbor model to compute the contribution of single mismatch to the thermodynamic of helix-coil transition. Either `"allsanpey"`, `"tur06"`, `"zno07"` `"zno08"` or `"wat11"` (see Single mismatch effect).
`method.tandemMM` | Specify the nearest neighbor model to compute the contribution of tandem mismatches to the thermodynamic of helix-coil transition. Either `"allsanpey"` or `"tur99"` (see Tandem mismatches effect).
`method.single.dangle` | Specify the nearest neighbor model to compute the contribution of single dangling end to the thermodynamic of helix-coil transition. Either `"bom00"`, `"sugdna02"`, `"sugrna02"` or `"ser08"` (see Single dangling end effect).
`method.double.dangle` | Specify the nearest neighbor model to compute the contribution of double dangling end to the thermodynamic of helix-coil transition. Either `"sugdna02"`, `"sugrna02"`, `"ser05"` or `"ser06"` (see Double dangling end effect).
`method.long.dangle` | Specify the nearest neighbor model to compute the contribution of long dangling end to the thermodynamic of helix-coil transition. Either `"sugdna02"` or `"sugrna02"` (see Long dangling end effect).
`method.internal.loop` | Specify the nearest neighbor model to compute the contribution of internal loop to the thermodynamic of helix-coil transition. Either `"san04"`, `"tur06"` or `"zno07"` (see Internal loop effect).
`method.single.bulge.loop` | Specify the nearest neighbor model to compute the contribution of single bulge loop to the thermodynamic of helix-coil transition. Either `"san04"`, `"tan04"`, `"ser07"` or `"tur06"` (see Single bulge loop effect).
`method.long.bulge.loop` | Specify the nearest neighbor model to compute the contribution of long bulge loop to the thermodynamic of helix-coil transition. Either `"san04"` or `"tur06"` (see Long bulge loop effect).
`method.CNG` | Specify the nearest neighbor model to compute the contribution of CNG repeats to the thermodynamic of helix-coil transition. Available method is `"bro05"` (see CNG repeats effect).
`method.inosine` | Specify the specific nearest neighbor model to compute the contribution of inosine bases (I) to the thermodynamic of helix-coil transition. Either `"san05"` or `"zno07"` (see Inosine bases effect).
`method.hydroxyadenine` | Specify the nearest neighbor model to compute the contribution of hydroxyadenine bases (A*) to the thermodynamic of helix-coil transition. Available method is `"sug01"` (see Hydroxyadenine bases effect).
`method.azobenzenes` | Specify the nearest neighbor model to compute the contribution of azobenzenes (X_T for trans azobenzenes and X_C for cis azobenzenes) to the thermodynamic of helix-coil transition. Available method is `"asa05"` (see Azobenzenes effect).
`method.locked` | Specify the nearest neighbor model to compute the contribution of locked nucleic acids (AL, GL, TL and CL) to the thermodynamic of helix-coil transition. Available method is `"mct04"` (see Locked nucleic acids effect).
`correction.ion` | Specify the correction method for ions. Either one of the following: Na corrections`"ahs01"`, `"kam71"`, `"owc1904"`, `"owc2004"`, `"owc2104"`, `"owc2204"`, `"san96"`, `"san04"`, `"schlif"`, `"tanna06"`, `"wetdna91"`, `"tanna07"`, `"wetrna91"` or `"wetdnarna91"` (see Sodium corrections) Mg corrections`"owcmg08"`, `"tanmg06"` or `"tanmg07"` (see Magnesium corrections) Mixed Na Mg corrections`"owcmix08"`, `"tanmix07"` or `"tanmix07"` (see Mixed Sodium and Magnesium corrections) .
`method.Naeq` | Specify the ion correction which gives a sodium equivalent concentration if other cations are present. Either `"ahs01"`, `"mit96"` or `"pey00"` (see Sodium equivalent concentration methods).
`correction.DMSO` | Specify the correction method for DMSO. Specify the correction method for DMSO. Either `"ahs01"`, `"mus81"`, `"cul76"` or `"esc80"` (see DMSO corrections).
`correction.formamide` | Specify the correction method for formamide. Specify the correction method for formamide Either `"bla96"` or `"lincorr"` (see Formamide corrections).

### Value

A list with the following components:

``Environment`` | A list with details about the melting temperature computation environment.
``Options`` | A list with details about the options (default or user specified) used for melting temperature computation.
``Results`` | A list with the results of the melting temperature computation including the enthalpy and entropy in case of nearest neighbour methods.
``Message`` | Error and/or Warning messages, if any.

### Mandatory arguments

The following are the arguments which are mandatory for computation.

`sequence`

  5' to 3' sequence of one strand of the nucleic acid duplex as a character string. Recognises A, C, G, T, U, I, X_C, X_T, A*, AL, TL, GL and CL. U and T are not considered identical (see Recognized nucleotides).

`comp.sequence`

  Mandatory if there are mismatches, inosine(s) or hydroxyadenine(s) between the two strands. If not specified, it is computed as the complement of `sequence`. Self-complementarity in `sequence` is detected even though there may be (are) dangling end(s) and `comp.sequence` is computed (see Self complementary sequences).

`nucleic.acid.conc`

  See Correction factor for nucleic acid concentration.

`Na.conc, Mg.conc, Tris.conc, K.conc`

  At least one cation (Na, Mg, Tris, K) concentration is mandatory, the other agents(dNTP, DMSO, formamide) are optional (see Ion and agent concentrations).

`hybridisation.type`

  See Hybridisation type options.

### Recognized nucleotides

Code | Type
A | Adenine
C | Cytosine
G | Guanine
T | Thymine
U | Uracil
I | Inosine
X_C | Trans azobenzenes
X_T | Cis azobenzenes
A* | Hydroxyadenine
AL | Locked nucleic acid
TL | "
GL | "
CL | "

U and T are not considered identical.

### Hybridisation type options

The details of the possible options for hybridisation type specified in the argument `hybridisation.type` are as follows:

Option | `Sequence` | `Complementary sequence`
`dnadna` | DNA | DNA
`rnarna` | RNA | RNA
`dnarna` | DNA | RNA
`rnadna` | RNA | DNA
`mrnarna` | 2-o-methyl RNA | RNA
`rnamrna` | RNA | 2-o-methyl RNA

This parameter determines the nature of the sequences in the arguments `sequence` and `comp.sequence`.

### Ion and agent concentrations

Ion concentrations are specified by the arguments `Na.conc`, `Mg.conc`, `Tris.conc` and `K.conc`, while agent concentrations are specified by the arguments `dNTP.conc`, `DMSO.conc` and `formamide.conc`.

These values are used for different correction functions which approximately adjusts for effects of these ions (Na, Mg, Tris, K) and/or agents (dNTP, DMSO, formamide) on on thermodynamic stability of nucleic acid duplexes. Their concentration limits depends on the correction method used. All the concentrations must be in M, except for the DMSO (%) and formamide (% or M depending on the correction method). Note that [Tris+] is about half of the total tris buffer concentration.

### Self complementary sequences

Self complementarity for perfect matching sequences or sequences with dangling ends is detected automatically. However it can be enforced by the argument `force.self = TRUE`.

### Correction factor for nucleic acid concentration

For self complementary sequences (Auto detected or specified by `force.self`) it is 1. Otherwise it is 4 if the both strands are present in equivalent amount and 1 if one strand is in excess.

### Approximative estimation formulas

Formula | Type | Limits/Remarks | Reference
`ahs01` | DNA | No mismatch | von Ahsen et al., 2001
`che93` | DNA | No mismatch; Na=0, Mg=0.0015, | Marmur and Doty, 1962
|  | Tris=0.01, K=0.05 |
`che93corr` | DNA | No mismatch; Na=0, Mg=0.0015, | Marmur and Doty, 1962
|  | Tris=0.01, K=0.05 |
`schdot` | DNA | No mismatch | Wetmur, 1991; Marmur and
|  |  | Doty, 1962; Chester and
|  |  | Marshak, 1993; Schildkraut
|  |  | and Lifson, 1965; Wahl et
|  |  | al., 1987; Britten et al.,
|  |  | 1974; Hall et al., 1980
`owe69` | DNA | No mismatch | Owen et al., 1969;
|  |  | Frank-Kamenetskii, 1971;
|  |  | Blake, 1996; Blake and
|  |  | Delcourt, 1998
`san98` | DNA | No mismatch | SantaLucia, 1998; von Ahsen
|  |  | et al., 2001
`wetdna91`* | DNA |  | Wetmur, 1991
`wetrna91`* | RNA |  | Wetmur, 1991
`wetdnarna91`* | DNA/RNA |  | Wetmur, 1991

* Default formula for computation.

Note that calculation is increasingly incorrect when the length of the duplex decreases. Further, it does not take into account nucleic acid concentration.

### Nearest neighbor models

#### Perfectly matching sequences

Model | Type | Limits/Remarks | Reference
`all97`* | DNA |  | Allawi and SantaLucia, 1997
`tur06`* | 2'-O-MeRNA/ | A sodium correction | Kierzek et al., 2006
| RNA | (`san04`) is |
|  | automatically applied to |
|  | convert the entropy (Na = |
|  | 0.1M) into the entropy (Na = |
|  | 1M). |
`bre86` | DNA |  | Breslauer et al., 1986
`san04` | DNA |  | SantaLucia and Hicks, 2004
`san96` | DNA |  | SantaLucia et al., 1996
`sug96` | DNA |  | Sugimoto et al., 1996
`tan04` | DNA |  | Tanaka et al., 2004
`fre86` | RNA |  | Freier et al., 1986
`xia98`* | RNA |  | Xia et al., 1998
`sug95`* | DNA/ |  | SantaLucia et al., 1996
| RNA |  |

* Default model for computation.

#### GU wobble base pairs effect

Model | Type | Limits/Remarks | Reference
`tur99` | RNA |  | Mathews et al., 1999
`ser12`* | RNA |  | Chen et al., 2012

* Default model for computation.

GU base pairs are not taken into account by the approximative mode.

#### Single mismatch effect

Model | Type | Limits.Remarks | Reference
`allsanpey`* | DNA |  | Allawi and SantaLucia, 1997;
|  |  | Allawi and SantaLucia, 1998;
|  |  | Allawi and SantaLucia, 1998;
|  |  | Allawi and SantaLucia, 1998;
|  |  | Peyret et al., 1999
`wat11`* | DNA/RNA |  | Watkins et al., 2011
`tur06` | RNA |  | Lu et al., 2006
`zno07`* | RNA |  | Davis and Znosko, 2007
`zno08` | RNA | At least one adjacent GU base | Davis and Znosko, 2008
|  | pair. |

* Default model for computation.

Single mismatches are not taken into account by the approximative mode.

#### Tandem mismatches effect

Model | Type | Limits.Remarks | Reference
`allsanpey`* | DNA | Only GT mismatches and TA/TG | Allawi and SantaLucia, 1997;
|  | mismatches. | Allawi and SantaLucia, 1998;
|  |  | Allawi and SantaLucia, 1998;
|  |  | Allawi and SantaLucia, 1998;
|  |  | Peyret et al., 1999
`tur99`* | RNA | No adjacent GU or UG base | Mathews et al., 1999; Lu et
|  | pairs. | al., 2006

* Default model for computation.

Tandem mismatches are not taken into account by the approximative mode. Note that not all the mismatched Crick's pairs have been investigated.

#### Single dangling end effect

Model | Type | Limits.Remarks | Reference
`bom00`* | DNA |  | Bommarito et al., 2000
`sugdna02` | DNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |
`sugrna02` | RNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |
`ser08`* | RNA | Only 3' UA, GU and UG | O'Toole et al., 2006; Miller
|  | terminal base pairs only 5' | et al., 2008
|  | UG and GU terminal base |
|  | pairs. |

* Default model for computation.

Single dangling ends are not taken into account by the approximative mode.

#### Double dangling end effect

Model | Type | Limits/Remarks | Reference
`sugdna02`* | DNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |
`sugrna02` | RNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |
`ser05` | RNA | Depends on the available | O'Toole et al., 2005
|  | thermodynamic parameters for |
|  | single dangling end. |
`ser06`* | RNA |  | O'Toole et al., 2006

* Default model for computation.

Double dangling ends are not taken into account by the approximative mode.

#### Long dangling end effect

Model | Type | Limits/Remarks | Reference
`sugdna02`* | DNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |
`sugrna02`* | RNA | Only terminal poly A self | Ohmichi et al., 2002
|  | complementary sequences. |

* Default model for computation.

Long dangling ends are not taken into account by the approximative mode.

#### Internal loop effect

Model | Type | Limits.Remarks | Reference
`san04`* | DNA | Missing asymmetry penalty. | SantaLucia and Hicks, 2004
|  | Not tested with experimental |
|  | results. |
`tur06` | RNA | Not tested with experimental | Lu et al., 2006
|  | results. |
`zno07`* | RNA | Only for 1x2 loop. | Badhwar et al., 2007

* Default model for computation.

Internal loops are not taken into account by the approximative mode.

#### Single bulge loop effect

Model | Type | Limits/Remarks | Reference
`tan04`* | DNA |  | Tan and Chen, 2007
`san04` | DNA | Missing closing AT penalty. | SantaLucia and Hicks, 2004
`ser07` | RNA | Less reliable results. Some | Blose et al., 2007
|  | missing parameters. |
`tur06`* | RNA |  | Lu et al., 2006

* Default model for computation.

Single bulge loops are not taken into account by the approximative mode.

#### Long bulge loop effect

Model | Type | Limits.Remarks | Reference
`san04`* | DNA | Missing closing AT penalty. | SantaLucia and Hicks, 2004
`tur06`* | RNA | Not tested with experimental | Mathews et al., 1999; Lu et
|  | results. | al., 2006

* Default model for computation.

Long bulge loops are not taken into account by the approximative mode.

#### CNG repeats effect

Model | Type | Limits/Remarks | Reference
`bro05`* | RNA | Self complementary sequences. | Broda et al., 2005
|  | 2 to 7 CNG repeats. |

* Default model for computation.

CNG repeats are not taken into account by the approximative mode. The contribution of CNG repeats to the thermodynamic of helix-coil transition can be computed only for 2 to 7 CNG repeats. N represents a single mismatch of type N/N.

#### Inosine bases effect

Model | Type | Limits/Remarks | Reference
`san05`* | DNA | Missing parameters for tandem | Watkins and SantaLucia, 2005
|  | base pairs containing inosine |
|  | bases. |
`zno07`* | RNA | Only IU base pairs. | Wright et al., 2007

* Default model for computation.

Inosine bases (I) are not taken into account by the approximative mode.

#### Hydroxyadenine bases effect

Model | Type | Limits/Remarks | Reference
`sug01`* | DNA | Only 5' GA*C 3'and 5' TA*A 3' | Kawakami et al., 2001
|  | contexts. |

* Default model for computation.

Hydroxyadenine bases (A*) are not taken into account by the approximative mode.

#### Azobenzenes effect effect

Model | Type | Limits/Remarks | Reference
`asa05`* | DNA | Less reliable results when | Asanuma et al., 2005
|  | the number of cis azobenzene |
|  | increases. |

* Default model for computation.

Azobenzenes (X_T for trans azobenzenes and X_C for cis azobenzenes) are not taken into account by the approximative mode.

#### Locked nucleic acids effect

Model | Type | Limits/Remarks | Reference
`mct04`* | DNA |  | McTigue et al., 2004

* Default model for computation.

Locked nucleic acids (AL, GL, TL and CL) are not taken into account by the approximative mode.

### Ion corrections

#### Sodium corrections

Correction | Type | Limits.Remarks | Reference
`ahs01` | DNA | Na>0. | von Ahsen et al., 2001
`schlif` | DNA | Na>=0.07; Na<=0.12. | Schildkraut and Lifson, 1965
`tanna06` | DNA | Na>=0.001; Na<=1. | Tan and Chen, 2006
`tanna07`* | RNA | Na>=0.003; Na<=1. | Tan and Chen, 2007
| or |  |
| 2'-O-MeRNA/RNA |  |
`wet91` | RNA, | Na>0. | Wetmur, 1991
| DNA |  |
| and |  |
| RNA/DNA |  |
`kam71` | DNA | Na>0; Na>=0.069; Na<=1.02. | Frank-Kamenetskii, 1971
`marschdot` | DNA | Na>=0.069; Na<=1.02. | Marmur and Doty, 1962; Blake
|  |  | and Delcourt, 1998
`owc1904` | DNA | Na>0. (equation 19) | Owczarzy et al., 2004
`owc2004` | DNA | Na>0. (equation 20) | Owczarzy et al., 2004
`owc2104` | DNA | Na>0. (equation 21) | Owczarzy et al., 2004
`owc2204`* | DNA | Na>0. (equation 22) | Owczarzy et al., 2004
`san96` | DNA | Na>=0.1. | SantaLucia et al., 1996
`san04` | DNA | Na>=0.05; Na<=1.1; | SantaLucia and Hicks, 2004;
|  | Oligonucleotides inferior to | SantaLucia, 1998
|  | 16 bases. |

* Default correction method for computation.

#### Magnesium corrections

Correction | Type | Limits/Remarks | Reference
`owcmg08`* | DNA | Mg>=0.0005; Mg<=0.6. | Owczarzy et al., 2008
`tanmg06` | DNA | Mg>=0.0001; Mg<=1; Oligomer | Tan and Chen, 2006
|  | length superior to 6 base |
|  | pairs. |
`tanmg07`* | RNA | Mg>=0.1; Mg<=0.3. | Tan and Chen, 2007

* Default correction method for computation.

#### Mixed Sodium and Magnesium corrections

Correction | Type | Limits.Remarks | Reference
`owcmix08`* | DNA | Mg>=0.0005; Mg<=0.6; | Owczarzy et al., 2008
|  | Na+K+Tris/2>0. |
`tanmix07` | DNA, | Mg>=0.1; Mg<=0.3; | Tan and Chen, 2007
| RNA | Na+K+Tris/2>=0.1; |
| or | Na+K+Tris/2<=0.3. |
| 2'-O-MeRNA/RNA |  |

* Default correction method for computation.

The ion correction by Owczarzy et al. (2008) is used by default according to the [Mg2+]0.5 ⁄ [Mon+] ratio, where [Mon+] = [Na+] + [Tris+] + [K+] .

If,

[Mon+] = 0

  Default sodium correction is used.

Ratio < 0.22,

  Default sodium correction is used.

0.22 <= Ratio < 6

  Default mixed Na and Mg correction is used.

Ratio >= 6

  Default magnesium correction is used.

Note that [Tris+] is about half of the total tris buffer concentration.

#### Sodium equivalent concentration methods

Correction | Type | Limits/Remarks | Reference
`ahs01`* | DNA |  | von Ahsen et al., 2001
`mit96` | DNA |  | Mitsuhashi, 1996
`pey00` | DNA |  | Peyret, 2000

* Default correction method for computation.

For the other types of hybridization, the DNA default correction is used. If there are other cations when an approximative approach is used, a sodium equivalence is automatically computed. In case of nearest neighbor approach, the sodium equivalence will be used only if a sodium correction is specified by the argument `correction.ion`.

### Denaturing agent corrections

#### DMSO corrections

Correction | Type | Limits/Remarks | Reference
`ahs01*` | DNA | Not tested with experimental | von Ahsen et al., 2001
|  | results. |
`cul76` | DNA | Not tested with experimental | Cullen and Bick, 1976
|  | results. |
`esc80` | DNA | Not tested with experimental | Escara and Hutton, 1980
|  | results. |
`mus81` | DNA | Not tested with experimental | Musielski et al., 1981
|  | results. |

* Default correction method for computation.

For the other types of hybridization, the DNA default correction is used. If there is DMSO when an approximative approach is used, a DMSO correction is automatically computed. In case of nearest neighbor approach and approximative approach, the DMSO correction will be used only if a sodium correction is specified by the argument `correction.ion`.

#### Formamide corrections

Correction | Type | Limits/Remarks | Reference
`bla96*` | DNA | With formamide concentration | Blake, 1996
|  | in mol/L. |
`lincorr` | DNA | With a formamide volume. | McConaughy et al., 1969;
|  |  | Record, 1967; Casey and
|  |  | Davidson, 1977; Hutton, 1977

* Default correction method for computation.

For the other types of hybridization, the DNA default correction is used. If there is formamide when an approximative approach is used, a formamide correction is automatically computed. In case of nearest neighbor approach and approximative approach, the formamide correction will be used only if a sodium correction is specified by the argument `correction.ion`.

### References

Marmur J, Doty P (1962). “Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.” Journal of Molecular Biology, 5(1), 109–118.

Schildkraut C, Lifson S (1965). “Dependence of the melting temperature of DNA on salt concentration.” Biopolymers, 3(2), 195–208.

Record MT (1967). “Electrostatic effects on polynucleotide transitions. I. Behavior at neutral pH.” Biopolymers, 5(10), 975–992.

McConaughy BL, Laird C, McCarthy BJ (1969). “Nucleic acid reassociation in formamide.” Biochemistry, 8(8), 3289–3295.

Owen R, Hill L, Lapage S (1969). “Determination of DNA base compositions from melting profiles in dilute buffers.” Biopolymers, 7(4), 503–516.

Frank-Kamenetskii MD (1971). “Simplification of the empirical relationship between melting temperature of DNA, its GC content and concentration of sodium ions in solution.” Biopolymers, 10(12), 2623–2624.

Britten RJ, Graham DE, Neufeld BR (1974). “Analysis of repeating DNA sequences by reassociation.” Methods in Enzymology, 29, 363–418.

Cullen BR, Bick MD (1976). “Thermal denaturation of DNA from bromodeoxyuridine substituted cells.” Nucleic Acids Research, 3(1), 49–62.

Hutton JR (1977). “Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea.” Nucleic Acids Research, 4(10), 3537–3555.

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### See Also

For more details about algorithm, formulae and methods, see the documentation for MELTING 5.

### Examples

    # Basic usage
    melting(sequence = "CAGTGAGACAGCAATGGTCG", nucleic.acid.conc = 2e-06,
            hybridisation.type = "dnadna", Na.conc = 1)

    # For more detailed examples refer the vignette.
    ## Not run:

    browseVignettes(package = 'rmelting')

    ## End(Not run)

[Package rmelting version 1.2.0 Index]