libScales {riboSeqR} | R Documentation

## Generates library scaling factors from ribosome footprint (and associated RNA) data.

### Description

This function applies the library scaling factor estimation methods implemented in the baySeq package to ribosome footprint data and (if present) the associated RNA-Seq data. The library scaling factors can then be used in downstream analyses.

### Usage

    libScales(rC, riboDat, lengths, frames, method = "edgeR")

### Arguments

`rC` | A `riboCoding` object containing the coordinates of the coding sequences and the number of ribosomal footprint reads of various classes to be found in each.
`riboDat` | A `riboData` object containing the ribosome footprint and RNA-seq alignments.
`lengths` | Lengths of ribosome footprints to inform count data.
`frames` | Frames of ribosome footprints (relative to coding start site). If omitted, all frames are used.
`method` | Method to be used for scaling factor estimation. See `getLibsizes`

### Value

A list object containing

`riboLS` | Ribosome footprint library scaling factors.
`rnaLS` | RNA-Seq library scaling factors.

### Author(s)

Thomas J. Hardcastle

[Package riboSeqR version 1.20.0 Index]