setBins {flowPloidy} | R Documentation

## setBins

### Description

(Re-)set the bins for a FlowHist object

### Usage

    setBins(fh, bins = 256)

### Arguments

`fh` | a `FlowHist` object
`bins` | integer, the number of bins to use in aggregating FCS data

### Details

This function sets (or resets) the number of bins to use in aggregating FCS data into a histogram, and generates the corresponding data matrix. Not exported for general use.

The `histData` matrix also contains the columns corresponding to the raw data used in calculating the single-cut and multiple-cut debris components, as well as the doublet, triplet, and quadruplet aggregate values. (i.e., `SCvals`, `MCvals`, `DBvals`, `TRvals`, and `QDvals`).

`setBins` includes a call to `resetFlowHist`, so all the model components that depend on the bins are updated in the process (as you want!).

### Value

a `FlowHist` object, with the `bins` slot set to `bins`, and the corresonding binned data stored in a matrix in the `histData` slot. Any previous analysis slots are removed: `peaks, comps, model, init, nls, counts, CV, RCS`.

### Author(s)

Tyler Smith

### Examples

    ## defaults to 256 bins:
    library(flowPloidyData)
    fh1 <- FlowHist(file = flowPloidyFiles()[1], channel = "FL3.INT.LIN")
    plot(fh1)
    ## reset them to 512 bins:
    fh1 <- setBins(fh1, 512)
    plot(fh1)

[Package flowPloidy version 1.12.0 Index]