genotype.Illumina {crlmm} | R Documentation

## Preprocessing and genotyping of Illumina Infinium II arrays.

### Description

Preprocessing and genotyping of Illumina Infinium II arrays.

### Usage

    genotype.Illumina(sampleSheet=NULL, arrayNames=NULL, ids=NULL, path=".",
          arrayInfoColNames=list(barcode="SentrixBarcode_A", position="SentrixPosition_A"),
          highDensity=FALSE, sep="_", fileExt=list(green="Grn.idat", red="Red.idat"), XY=NULL, anno, genome,
          call.method="crlmm", trueCalls=NULL, cdfName, copynumber=TRUE, batch=NULL, saveDate=FALSE, stripNorm=TRUE,
          useTarget=TRUE, quantile.method="between", nopackage.norm="quantile", mixtureSampleSize=10^5, fitMixture=TRUE,
          eps=0.1, verbose = TRUE, seed = 1, sns, probs = rep(1/3, 3), DF = 6, SNRMin = 5,
          recallMin = 10, recallRegMin = 1000, gender = NULL, returnParams = TRUE, badSNP = 0.7)

    crlmmIllumina(sampleSheet=NULL, arrayNames=NULL, ids=NULL, path=".",
          arrayInfoColNames=list(barcode="SentrixBarcode_A", position="SentrixPosition_A"),
          highDensity=FALSE, sep="_", fileExt=list(green="Grn.idat", red="Red.idat"), XY=NULL, anno, genome,
          call.method="crlmm", trueCalls=NULL, cdfName, copynumber=TRUE, batch=NULL, saveDate=FALSE, stripNorm=TRUE,
          useTarget=TRUE, quantile.method="between", nopackage.norm="quantile", mixtureSampleSize=10^5, fitMixture=TRUE,
          eps=0.1, verbose = TRUE, seed = 1, sns, probs = rep(1/3, 3), DF = 6, SNRMin = 5,
          recallMin = 10, recallRegMin = 1000, gender = NULL, returnParams = TRUE, badSNP = 0.7)

### Arguments

`sampleSheet` | `data.frame` containing Illumina sample sheet information (for required columns, refer to BeadStudio Genotyping guide - Appendix A).
`arrayNames` | character vector containing names of arrays to be read in. If `NULL`, all arrays that can be found in the specified working directory will be read in.
`ids` | vector containing ids of probes to be read in. If `NULL` all probes found on the first array are read in.
`path` | character string specifying the location of files to be read by the function
`arrayInfoColNames` | (used when `sampleSheet` is specified) list containing elements 'barcode' which indicates column names in the `sampleSheet` which contains the arrayNumber/barcode number and 'position' which indicates the strip number. In older style sample sheets, this information is combined (usually in a column named 'SentrixPosition') and this should be specified as `list(barcode=NULL, position="SentrixPosition")`
`highDensity` | logical (used when `sampleSheet` is specified). If `TRUE`, array extensions '\_A', '\_B' in sampleSheet are replaced with 'R01C01', 'R01C02' etc.
`sep` | character string specifying separator used in .idat file names.
`fileExt` | list containing elements 'Green' and 'Red' which specify the .idat file extension for the Cy3 and Cy5 channels.
`XY` | `NChannelSet` containing X and Y intensities.
`anno` | data.frame containing SNP annotation information from manifest and additional columns 'isSnp', 'position', 'chromosome' and 'featureNames'. For use when `cdfName`='nopackage'
`genome` | character string specifying which genome is used in annotation
`call.method` | character string specifying the genotype calling algorithm to use ('crlmm' or 'krlmm').
`trueCalls` | matrix specifying known Genotype calls(can contain some NAs) for a subset of samples and features (1 - AA, 2 - AB, 3 - BB).
`cdfName` | annotation package (see also `validCdfNames`) or 'nopackage' when combined with 'krlmm', an `anno` data.frame and `genome`.
`copynumber` | 'logical.' Whether to store copy number intensities with SNP output.
`batch` | character vector indicating the batch variable. Must be the same length as the number of samples. See details.
`saveDate` | 'logical'. Should the dates from each .idat be saved with sample information?
`stripNorm` | 'logical'. Should the data be strip-level normalized?
`useTarget` | 'logical' (only used when `stripNorm=TRUE`). Should the reference HapMap intensities be used in strip-level normalization?
`quantile.method` | character string specifying the quantile normalization method to use ('within' or 'between' channels).
`nopackage.norm` | character string specifying normalization to be used when `cdfName`='nopackage'. Options are 'none', 'quantile' (within channel, between array) and 'loess'.
`mixtureSampleSize` | Sample size to be use when fitting the mixture model.
`fitMixture` | 'logical.' Whether to fit per-array mixture model.
`eps` | Stop criteria.
`verbose` | 'logical.' Whether to print descriptive messages during processing.
`seed` | Seed to be used when sampling. Useful for reproducibility
`sns` | The sample identifiers. If missing, the default sample names are `basename(filenames)`
`probs` | 'numeric' vector with priors for AA, AB and BB.
`DF` | 'integer' with number of degrees of freedom to use with t-distribution.
`SNRMin` | 'numeric' scalar defining the minimum SNR used to filter out samples.
`recallMin` | Minimum number of samples for recalibration.
`recallRegMin` | Minimum number of SNP's for regression.
`gender` | integer vector ( male = 1, female = 2 ) or missing, with same length as filenames. If missing, the gender is predicted.
`returnParams` | 'logical'. Return recalibrated parameters from crlmm.
`badSNP` | 'numeric'. Threshold to flag as bad SNP (affects batchQC)

### Details

`genotype.Illumina` (or equivalently `crlmmIllumina`) is a wrapper of the `crlmm` function for genotyping. Differences include (1) that the copy number probes (if present) are also quantile-normalized and (2) the class of object returned by this function, `CNSet`, is needed for subsequent copy number estimation. Note that the batch variable (a character string) has no effect on the normalization or genotyping steps. Rather, `batch` is required in order to initialize a `CNSet` container with the appropriate dimensions.

The new 'krlmm' option is available for certain chip types. Optional argument `trueCalls` matrix contains known Genotype calls (1 - AA, 2 - AB, 3 - BB) for a subset of samples and features. This will used to compute KRLMM coefficients by calling `vglm` function from `VGAM` package.

The 'krlmm' method makes use of functions provided in `parallel` package to speed up the process. It by default initialises up to 8 clusters. This is configurable by setting up an option named "krlmm.cores", e.g. options("krlmm.cores" = 16).

In general, a chip specific annotation package is required to use the `genotype.Illumina` function. If this is not available (newer chip types or custom chips often don't have a chip-specific package available on Bioconductor), consider using `cdfName`='nopackage' and specifying `anno` and `genome`, which runs 'krlmm' on the samples available. Here `anno` is a data.frame read in from the relevant chip-specific manifest, which must have additional columns 'isSnp' which is a logical that indicates whether a probe is polymorphic or not, 'position', 'chromosome' and 'featureNames' that give the location on the chromosome and SNP name.

### Value

A `SnpSuperSet` instance.

### Author(s)

Matt Ritchie, Cynthia Liu, Zhiyin Dai

### References

Ritchie ME, Carvalho BS, Hetrick KN, Tavar\'e S, Irizarry RA. R/Bioconductor software for Illumina's Infinium whole-genome genotyping BeadChips. Bioinformatics. 2009 Oct 1;25(19):2621-3.

Liu R, Dai Z, Yeager M, Irizarry RA1, Ritchie ME. KRLMM: an adaptive genotype calling method for common and low frequency variants. BMC Bioinformatics. 2014 May 23;15:158.

Carvalho B, Bengtsson H, Speed TP, Irizarry RA. Exploration, normalization, and genotype calls of high-density oligonucleotide SNP array data. Biostatistics. 2007 Apr;8(2):485-99. Epub 2006 Dec 22. PMID: 17189563.

Carvalho BS, Louis TA, Irizarry RA. Quantifying uncertainty in genotype calls. Bioinformatics. 2010 Jan 15;26(2):242-9.

### See Also

`ocSamples`, `ldOpts`

### Examples

    ## Not run:
    	# example for 'crlmm' option
    	library(ff)
    	library(crlmm)
    	## to enable paralellization, set to TRUE
    	if(FALSE){
    		library(snow)
    		library(doSNOW)
    		## with 10 workers
    		cl <- makeCluster(10, type="SOCK")
    		registerDoSNOW(cl)
    	}
    	## path to idat files
    	datadir <- "/thumper/ctsa/snpmicroarray/illumina/IDATS/370k"
    	## read in your samplesheet
    	samplesheet = read.csv(file.path(datadir, "HumanHap370Duo_Sample_Map.csv"), header=TRUE, as.is=TRUE)
    	samplesheet <- samplesheet[-c(28:46,61:75,78:79), ]
    	arrayNames <- file.path(datadir, unique(samplesheet[, "SentrixPosition"]))
    	arrayInfo <- list(barcode=NULL, position="SentrixPosition")
    	cnSet <- genotype.Illumina(sampleSheet=samplesheet,
    				   arrayNames=arrayNames,
    				   arrayInfoColNames=arrayInfo,
    				   cdfName="human370v1c",
    				   batch=rep("1", nrow(samplesheet)))

    ## End(Not run)
    ## Not run:
    	# example for 'krlmm' option
    	library(crlmm)
    	library(ff)
    	# line below is an optional step for krlmm to initialise 16 workers
    	# options("krlmm.cores" = 16)
    	# read in raw X and Y intensities output by GenomeStudio's GenCall genotyping module
    	XY = readGenCallOutput(c("HumanOmni2-5_4v1_FinalReport_83TUSCAN.csv","HumanOmni2-5_4v1_FinalReport_88CHB-JPT.csv"),
    				cdfName="humanomni25quadv1b",
    				verbose=TRUE)
    	krlmmResult = genotype.Illumina(XY=XY,
    		      			cdfName=ThiscdfName,
    					call.method="krlmm",
    					verbose=TRUE)

    	# example for 'krlmm' option with known genotype call for some SNPs and samples
    	library(VGAM)
    	hapmapCalls = load("hapmapCalls.rda")
    	# hapmapCalls should have rownames and colnames corresponding to XY featureNames and sampleNames
    	krlmmResult = genotype.Illumina(XY=XY,
    					cdfName=ThiscdfName,
    					call.method="krlmm",
    					trueCalls=hapmapCalls,
    					verbose=TRUE)

    ## End(Not run)

[Package crlmm version 1.44.0 Index]