simReads {casper} | R Documentation

## Function to simulate paired end reads following given read start and fragment length distributions and gene and variant expressions.

### Description

This function generates path counts and bam files with simulated paired end reads according to given read start distribution, fragment length distribution and gene and variant expressions.

### Usage

    simReads(islandid, nSimReads, pis, rl, seed, writeBam, distrs, genomeDB,
    repSims=FALSE, bamFile=NULL, stranded=FALSE, verbose=TRUE, chr=NULL, mc.cores=1)

### Arguments

`islandid` | Island ID's from the genomeDB object to simulate reads
`nSimReads` | Named numeric vector with number of fragments to simulate in each island.
`pis` | Named numeric vector with relative expression of transcripts. Expressions add up to one for each island to simulate.
`rl` | Read length
`seed` | Seed of the random numbers generator
`writeBam` | Set to 1 to generate bam files with the simulated reads
`distrs` | Object of class 'readDistrs' with read start and fragment length distributions
`genomeDB` | Object of class 'annotatedGenome' with the genome to genererate reads from
`repSims` | Set to TRUE to return relative read starts and fragment lengths from the simulation
`bamFile` | Name of the bam file to write reads to. Must end with '.bam'
`stranded` | Set to TRUE to preserve gene strand when generating reads. The 'XS' tag will be added to reads in the bam file and the returned 'pc' object will be stranded
`verbose` | Set to `TRUE` to print progress
`chr` | Characters vector with chromosomes to simulate. Defaults to whole genome simulations.
`mc.cores` | Number of cores to use in function

### Value

`Nsim` | Numerical vector with the number of reads simulated for each island.
`pc` | Object of class 'pathCounts' with simulated path counts
`sims` | Only if 'repSims' is set to TRUE. List with vectors of length 'n' with the following elements: -'varl': Length of variant for corresponding read -'st' Start of fragment relative to variant start (not in genomic coordinates) -len:Fragment length -'strand':Strand of gene for simulated read

### Author(s)

Camille Stephan-Otto Attolini

### Examples

    data(hg19DB)
    data(K562.r1l1)
    distrs <- getDistrs(hg19DB,bam=K562.r1l1,readLength=75)

    islandid <- c('10319','463')
    txs <- unlist(lapply(hg19DB@transcripts[islandid], names))
    pis <- vector(mode='numeric', length=length(txs))
    npis <- sapply(hg19DB@transcripts[islandid],length)
    pis[1:npis[1]] <- rep(1/npis[1],npis[1])
    pis[-1:-npis[1]] <- rep(1/npis[2],npis[2])
    names(pis) <- txs
    nSimReads <- c(100, 100)
    names(nSimReads) <- islandid

    simpc <- simReads(islandid=islandid, nSimReads=nSimReads, pis=pis,
    rl=75, repSims=TRUE, seed=1, writeBam=FALSE, distrs=distrs,genomeDB=hg19DB)

[Package casper version 2.20.0 Index]