normalize.quantile.450k {ENmix} | R Documentation

## Quantile normalization.

### Description

Quantile normalization of methylation intensity data across samples for Illumina Infinium HumanMethylation 450 BeadChip.

### Usage

    normalize.quantile.450k(mdat, method = "quantile1")

### Arguments

`mdat` | An object of class `MethylSet`.
`method` | Quantile normalization method. This should be one of the following strings: "quantile1", "quantile2", or "quantile3".

### Details

By default, method = "quantile1" will separately quantile normalize Methylated or Unmethylated intensities for Infinium I or II probes. The "quantile2" will quantile normalize combined Methylated or Unmethylated intensities for Infinium I or II probes. The "quantile3" will quantile normalize combined Methylated or Unmethylated intensities for Infinium I and II probes together.

### Value

An object of class `MethylSet`.

### Author(s)

Zongli Xu

### References

Pidsley, R., CC, Y.W., Volta, M., Lunnon, K., Mill, J. and Schalkwyk, L.C. (2013) A data-driven approach to preprocessing Illumina 450K methylation array data. BMC genomics, 14, 293.

### Examples

    if(FALSE){
    if (require(minfiData)) {
    mdat=preprocessENmix(RGsetEx,bgParaEst="oob",nCores=6)
    mdatq1=normalize.quantile.450k(mdat,method="quantile1")
    }}

[Package ENmix version 1.22.6 Index]