relQuantPCR              package:SLqPCR              R Documentation

_C_o_m_p_u_t_e _r_e_l_a_t_i_v_e _e_x_p_r_e_s_s_i_o_n _v_a_l_u_e_s _f_o_r _r_e_a_l_t_i_m_e _q_u_a_n_t_i_t_a_t_i_v_e _R_T-_P_C_R _d_a_t_a

_D_e_s_c_r_i_p_t_i_o_n:

     Compute relative expression values for realtime quantitative
     RT-PCR data based on  Ct or take-off values, respectively. The
     computations use the PCR efficiency.

_U_s_a_g_e:

     relQuantPCR(x, E = 2, na.rm = FALSE)

_A_r_g_u_m_e_n_t_s:

       x: numeric vector containing raw data 

       E: PCR efficiency 

   na.rm: a logical value indicating whether 'NA' values should be
          stripped before the computation proceeds.  

_V_a_l_u_e:

     vector of relative expression values w.r.t. specified PCR
     efficiency.

_A_u_t_h_o_r(_s):

     Dr. Matthias Kohl (SIRS-Lab GmbH) kohl@sirs-lab.com

_R_e_f_e_r_e_n_c_e_s:

     Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002).
     Accurate  normalization of real-time quantitative RT-PCR data by
     geometric averaging  of multiple internal control genes.  Genome
     Biology 2002. 3(7):research0034.1-0034.11. <URL:
     http://genomebiology.com/2002/3/7/research/0034/>

