hiv2raw {HIVcDNAvantWout03} | R Documentation

## T cell line infections with HIV-1 LAI (BRU)

### Description

The expression levels of approximately 4600 cellular RNA transcripts were assessed in CD4+ T cell lines at different times after infection with HIV-1BRU using DNA microarrays. There are two data sets, which are part of a dye-swap experiment with replicates, representing the Cy3 (green) absorption intensities for channel 1 (`hiv1raw`) and the Cy5 (red) absorption intensities for channel 2 (`hiv2raw`).

### Usage

    data(hiv2raw)

### Format

This data represents a block within a microarray image with 12x32 spots. It is stored as a vector of length 450,000 representing a 450x1000 matrix (ordered by column) of intensities encoded for compact (16-bit TIFF) storage.

### Details

The intensities can be obtained from this data by first subtracting them from 65535, then squaring, then multiplying by a scale factor 4.71542407E-05. In other words, a number `x` in the `hiv1` data set corresponds to intensity

    (256*256 - 1 - x)^2*.0000471542407

. \

### Source

Dr. Angelique van't Wout, Department of Microbiology, University of Washington\ The data corresponds to the first block of a 12 block array image (`001030_08_1.GEL`) in the first data set (`2000095918 A`) in the first experiment (`CEM LAI vs HI-LAI 24hr`) of the following data archive:\ http://expression.microslu.washington.edu/expression/vantwoutjvi2002.html

### References

{van't Wout} AB, Lehrman GK, Mikheeva SA, O'Keeffe GC, Katze MG, Bumgarner RE, Geiss GK, Mullins JI\ Cellular gene expression upon human immunodeficiency virus type 1 infection of CD4(+)-T-cell lines.\ J Virol. 2003 Jan;77(2):1392-402.

[Package HIVcDNAvantWout03 version 1.2.0 Index]