hiv1raw          package:HIVcDNAvantWout03          R Documentation

_T _c_e_l_l _l_i_n_e _i_n_f_e_c_t_i_o_n_s _w_i_t_h _H_I_V-_1 _L_A_I (_B_R_U)

_D_e_s_c_r_i_p_t_i_o_n:

     The expression levels of approximately  4600 cellular RNA
     transcripts were assessed in CD4+ T cell lines at different  times
     after infection with HIV-1BRU using DNA microarrays. There are two
     data sets, which are part of a dye-swap experiment with
     replicates, representing the Cy3 (green) absorption intensities
     for channel 1 ('hiv1raw') and the Cy5 (red) absorption intensities
     for channel 2 ('hiv2raw').

_U_s_a_g_e:

     data(hiv1raw)

_F_o_r_m_a_t:

     This data represents a block within a  microarray image with 12x32
     spots. It is stored as a vector of length 450,000 representing a
     450x1000 matrix (ordered by column) of intensities encoded for
     compact (16-bit TIFF) storage.

_D_e_t_a_i_l_s:

     The intensities can be obtained from this data by first 
     subtracting them from 65535, then squaring, then multiplying by  a
     scale factor 4.71542407E-05. In other words, a number 'x' in the
     'hiv1' data set corresponds to intensity   (256*256 - 1 -
     x)^2*.0000471542407 . \

_S_o_u_r_c_e:

     Dr. Angelique van't Wout, Department of Microbiology,  University
     of Washington\ The data corresponds to the first block of a 12
     block array image ('001030_08_1.GEL') in the first data set
     ('2000095918 A') in the first experiment ('CEM LAI vs HI-LAI
     24hr') of the following data archive:\ <URL:
     http://expression.microslu.washington.edu/expression/vantwoutjvi20
     02.html>

_R_e_f_e_r_e_n_c_e_s:

     {van't Wout} AB, Lehrman GK, Mikheeva SA, O'Keeffe GC, Katze MG,
     Bumgarner RE,  Geiss GK, Mullins JI\ Cellular gene expression upon
     human immunodeficiency virus type 1 infection of CD4(+)-T-cell
     lines.\ _J Virol._ 2003 Jan;77(2):1392-402.

